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Over the last few years, bacteria were subjected to many experimental procedures to evaluate how such unicellular systems may respond to EMFs. Inhan-Garip et al., 2011 studied the effect of extremely low frequency (<300 Hz) electromagnetic fields (ELF-EMF) on the growth rate of Gram-positive and Gram-negative bacteria and determined morphological changes that might have beenД¬aused by ELF-EMFs. In particular, it has been demonstrated that ELF-EMFs can negatively, or positively affect functional parameters (cell growth and viability). Fadel et al.,2003 reported that the main damaging role of 50 Hz magnetic field may be on the cellular membrane that strongly affects, not only the cellular physiological functions, but also the cell-to-cell communications.Д®ffects of ELF-EMFs on bacterial cells have been also studied.

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As a consequence, the effect of ELF-EMFs on the biological functions of living organisms represent an emerging area of interest with respect to environmental influences on human health.In latest years, several studies have been performed to study the variety of cell responses observed due to exposure to ELF-EMFs involving proliferation and differentiation ,gene expression^, 4],modulation of the membrane receptors functionality, apoptosis, alteration in ion homeostasis, and free radical generation. In the modern society, greater use of technologies leads to increasing exposure to extremely low frequency electromagnetic fields (ELF-EMFs) generated by structures and appliances such as power lines and ordinary devices used inside house and work places. Key words: Staphylococcus aureus, ELF-EMF, bacterial growth rate, antibiotic sensitivity, morphological changes, TEM, RAPD- PCR.

Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) revealed genetic fingerprinting variation as observed in the electrophoresis patterns between exposed and unexposed cells of S.aureus. Significant ultra structural changes occurred as observed by Transmission Electron Microscope (TEM). Moreover, exposed cells became more sensitive to the tested antibiotics compared to control. The results showed that a highly significant inhibition effect occurred when S.aureus was exposed to resonance of 0.8 Hz QAMW for a single exposure (120min). In order to compare cell viability, number of colony-forming units (CFU) and growth rate (optical density at 600nm) was determined. The present work aimed to study the biological effects of Extremely Low Frequency Electromagnetic Field (ELF-EMF) on the growth rate, morphology and antibiotic sensitivity patterns of the Gram-positive bacterium Staphylococcus aureus to determine any morphological and metabolic changes that might have been caused by ELF-EMF. A.dДЄBiophysics Department, Faculty of Science, Cairo University, Egypt bPhysics Department, Faculty of Science, Alexandria University, Egypt cBotany & Microbiology Department, Faculty of Science, Alexandria University, Egypt dPhysics Department, Faculty of Science, Damanhour University, Egypt E-mail: OF STAPHYLOCOCCUS AUREUS GROWTH BY ELECTROMAGNETIC THERAPY Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) revealed genetic fingerprinting variation as observed in the electrophoresis patterns between exposed and unexposed cells of S.aureus.ДЄli F. Significant ultra structural changes occurred as observed by Transmission Electron Microscope ( TEM).

The present work aimed to study the biological effects of Extremely Low Frequency Electromagnetic Field ( ELF-EMF) on the growth rate, morphology and antibiotic sensitivity patterns of the Gram-positive bacterium Staphylococcus aureus to determine any morphological and metabolic changes that might have been caused by ELF-EMF.